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  • BioPaddles

    Order Code BioPaddles

    ◾Ready to use. Saves time! ◾Longer shelf-life than traditional Petri dishes ◾No refrigeration required ◾Simple incubation requirements ◾Unique design enhances colony morphology characterization ◾Apps available for pressumtive ID's

    BioPaddles® are flexible dual-agar paddles each containing microbe-specific media enclosed in a sterile vial. Identify and quantify microbes in air, soil, water, or any surface! BioPaddles do not require any other testing equipment – only a magnifier and warm place (35°C or incubator) are needed.

    Liquid Sampling: Remove the paddle from the vial and fill the vial (approximately 40ml) with the sample. Insert the paddle, swirl for 15 seconds. Pour out the liquid, and replace the paddle in the vial and incubate.

    Surface Sampling: Remove the paddle from the vial and gently touch each paddle media surface to the sample surface twice per side. Replace paddle in the vial and incubate.

    Air Sampling: Remove the paddle from the vial. Invert and mount the circular cap into the vial, exposing the agar covered paddle. Expose for 15 minutes. Replace the paddle in the vial and incubate.

    All BioPaddle products include a free app! LaMotte BioPaddle Colony ID lite App allows users to compare the “unknown” microbe growth on the BioPaddle to a library of images of “known” microbe colonies from 5 microhabitats (air, water, soil, surface and food).

    New BioPaddle Colony ID full app now available! Click here to view the BioPaddle Apps.

    BioPaddle Products – all packaged 10 paddles per box. Includes a technical information sheet.

    Note: Dated material. Specify ship date. Orders may be placed in advance. Orders are shipped Mondays and Tuesdays from Maryland.

  • BioPaddles® - NA/NA

    Order Code 5550

    The BioPaddles® NA/NA can be used for the cultivation of a wide variety of non-fastidious bacteria.

    Nutrient Agar (NA) – The nitrogen, carbon, vitamins, and amino acids in Nutrient Agar are provided by enzymatic digest of gelatin and beef extract. Agar and a proprietary polymer are the solidifying agents.

    Application

    In the early 1900’s, the American Public Health Association (APHA) suggested the formula of Nutrient Agar as a standard culture medium used in water testing.1 Nutrient Agar continues to be a widely used general purpose medium for growing non-fastidious microorganisms.

    If required, enrichments can be added to this medium. Nutrient Agar meets APHA and Association of Official Analytical Chemists (AOAC) standard methods.Nutrient Agar is specified in Standard Methods for the Examination of Water and Wastewater procedures for the examination of food, dairy products, water, and other materials.

  • BioPaddles® - SAB/SAB

    Order Code 5551

    The BioPaddles® SAB/SAB can be used for the Selective isolation of fungi (yeasts and molds) (SAB).

    Sabouraud Dextrose Agar (SAB) – Enzymatic digest of casein and enzymatic digest of animal tissue provide the nitrogen and vitamin source required for organism growth in SAB. The high concentration of dextrose is included as an energy source. Agar and a proprietary polymer are the solidifying agents.

    Application

    Sabouraud Dextrose Agar (SAB) is a modification of dextrose agar described by Sabouraud. SAB is used for cultivating pathogenic and commensal fungi and yeasts. The high dextrose concentration and acidic pH of the formula permit selectivity of fungi. Sabouraud Dextrose Agar is used for determining the microbial content of cosmetics, in the mycological evaluation of food, and clinically to aid in the diagnosis of yeast and fungal infections.

  • BioPaddles® - TSA/RB

    Order Code 5552

    The BioPaddles® TSA/RB can be used for the cultivation of a wide variety of aerobic and anaerobic microorganisms (TSA). Selective enumeration and cultivation of yeasts, molds and Actinomycetes from food and other surfaces. (RB)

    Tryptic Soy Agar (TSA) – In 1955, Leavitt et al. discovered that Tryptic Soy Agar facilitated vigorous growth of aerobic and anaerobic microorganisms. This is an enriched media, suitable to support fastidious heterotrophs.

    Rose Bengal Chloramphenicol Agar (RB) – Selective medium for the enumeration of fungi. This formula is prepared with a neutral pH and supplemented with chloramphenicol as the selective agent in fungal medium. RB Agar is also referr

    Application

    Tryptic Soy Agar is recommended in multiple water & wastewater applications, and numerous standard methods for food testing. TSA is commonly used as a maintenance medium for culture collections and testing bacterial contaminants in cosmetics. Rose Bengal Chloramphenicol Agar is recommended in Standard Methods for the Examination of Water and Wastewater for the enumeration of yeasts and molds from foods and water.

  • BioPaddles® - NUT-TTC/MAC

    Order Code 5553

    Where to Buy

    Microbiological Testing ProductsBioPaddles Coliform Biological Activity Reaction Test

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    BioPaddles® - NUT-TTC/MAC Order Code: 5553

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    5553 200x200Nutrient-TTC Agar (NUT-TTC) – General purpose relatively non-selective agar medium, containing two peptones, which will support the growth of a wide variety of organisms. Suitable for cultivation of both of aerobes and anaerobes. This medium contains the dye, 2, 3, 5- triphenyltetrazolium chloride (TTC) . Aerobic coliform bacteria species grow on this medium and can be detected by their ability to reduce TTC to a red colored formazan dye. Bacterial colonies appear as red dots on an otherwise clear yellowish medium. Agar is the solidifying agent.

    MacConkey Agar (MAC) – Both selective and differential; used to differentiate between Gram negative bacteria while inhibiting the growth of most Gram positive bacteria. The medium also differentiates between lactose-fermenting coliforms Lac (+) and lactose non-fermenters Lac (-), which include potential pathogens. Addition to the nutrient agar base of bile salts and crystal violet will inhibit the growth of most Gram-positive bacteria, making MacConkey agar selective. Lactose, a fermentable carbohydrate, and neutral red, a pH indicator, are added to differentiate the lactose positive coliforms from the potentially pathogenic lactose non-fermenters. When lactose is fermented, acid products lower the pH below 6.8, with the resulting colonial growth turning pinkish-red. If an organism is unable to ferment lactose, the colonies will be colorless. Bile salts mixture and crystal violet are the selective agents, inhibiting Gram positive cocci and allowing Gram-negative organisms to grow. Sodium chloride maintains the osmotic environment. Agar and a proprietary polymer are the solidifying agents.

    Application

    In total coliform testing (TCC), the coliform organisms tested for include: total coliform, fecal coliform, and E. coli (Escherichia coli). Detection of fecal coliforms (a subset of total coliforms) or Escherichia coli (a subset of fecal coliforms) can indicate the potential presence of waterborne pathogens associated with fecal contamination.

  • BioPaddles® - R2A/MAC

    Order Code 5540

    The BioPaddles® R2A/MAC can be used for the enumeration and cultivation of bacteria from potable water; total coliform testing (TCC). Coliform Testing / Recovery of Stressed Coliforms.

    R2A Agar (R2A) – Developed by Reasoner and Geldreich for bacterial plate counts of treated potable water as well as oligotrophic (thrive in low-nutrient conditions) heterotrophs. R2A Agar, in combination with a lower incubation temperature and longer incubation time, stimulates the growth of stressed and chlorine-tolerant bacteria. Enzymatic digest of casein and animal tissue provides nitrogen, carbon and minerals in R2A Agar. Yeast extract is a source of vitamins and trace elements. Dextrose serves as a carbon source. Soluble starch aids in the recovery of injured organisms by absorbing toxic metabolic by-products. Dipotassium Phosphate is used to balance the pH, and magnesium sulfate heptahydrate is a source of divalent cations and sulfate. Sodium pyruvate increases the recovery of stressed cells. Agar and a proprietary polymer are the solidifying agents.

    MacConkey Agar (MAC) – Both selective and differential; used to differentiate between Gram negative bacteria while inhibiting the growth of most Gram positive bacteria. The medium also differentiates between lactose-fermenting coliforms Lac (+) and lactose non-fermenters Lac (-), which include potential pathogens. Addition to the nutrient agar base of bile salts and crystal violet will inhibit the growth of most Gram-positive bacteria, making MacConkey agar selective. Lactose, a fermentable carbohydrate, and neutral red, a pH indicator, are added to differentiate the lactose positive coliforms from the potentially pathogenic lactose non-fermenters. When lactose is fermented, acid products lower the pH below 6.8, with the resulting colonial growth turning pinkish-red. If an organism is unable to ferment lactose, the colonies will be colorless. Bile salts mixture and crystal violet are the selective agents, inhibiting Gram positive cocci and allowing Gram negative organisms to grow. Sodium Chloride maintains the osmotic environment. Agar and a proprietary polymer are the solidifying agents.

    Application

    For bacterial plate counts of treated potable water as well as oligotrophic heterotrophs. In total coliform testing (TCC), the coliform organisms tested for include: total coliform, fecal coliform, and E. coli (Escherichia coli). Detection of fecal coliforms (a subset of total coliforms) or Escherichia coli (a subset of fecal coliforms) can indicate the potential presence of waterborne pathogens associated with fecal contamination. R2A Agar is recommended in Standard Methods for the Examination of Water and Wastewater for pour plate, spread plate and membrane filter methods for heterotrophic counts.

  • BioPaddles® - NUT-TTC/EMB

    Order Code 5541

    The BioPaddles® NUT-TTC/EMB can be used for the isolation and differentiation of Gram (-) enteric bacilli. Coliform Testing / Recovery of Stressed Coliforms.

    Nutrient-TTC Agar (NUT-TTC) – General purpose, relatively non-selective agar medium, containing two peptones, which will support the growth of a wide variety of organisms. Suitable for cultivation of both aerobes and anaerobes. This medium contains the dye, 2, 3 ,5- triphenyltetrazolium chloride (TTC) . Aerobic coliform bacteria species grow on this medium and they can be detected by their ability to reduce TTC to a red colored formazan dye. Bacterial colonies appear as red dots on an otherwise clear (yellowish) medium. Gram (+) bacteria are inhibited. Agar and proprietary polymers are used as solidifying agents.

    Eosin Methylene Blue Agar-Levine (EMB) - Differential and slightly selective medium for the isolation, cultivation and differentiation of Gram negative enteric microorganisms (bacilli) isolated from both clinical and nonclinical specimens. Gram (+) bacteria are inhibited. It is widely used for the examination of materials for the presence of coliforms.

    Application

    In total coliform testing (TCC), the coliform organisms tested for include: total coliform, fecal coliform, and E. coli (Escherichia coli). Detection of fecal coliforms (a subset of total coliforms) or Escherichia coli (a subset of fecal coliforms) can indicate the potential presence of waterborne pathogens associated with fecal contamination.

  • BioPaddles® - MAC/EMB

    Order Code 5544

    The BioPaddles® MAC/EMB can be used for the isolation and differentiation of Gram (-) enteric bacilli (MAC) / Coliform Testing / Recovery of Stressed Coliforms (EMB).

    MacConkey Agar (MAC) – Both selective and differential; used to differentiate between Gram negative bacteria while inhibiting the growth of most Gram positive bacteria. The medium also differentiates between lactose-fermenting coliforms Lac (+) and lactose non-fermenters Lac (-), which include potential pathogens. Addition to the nutrient agar base of bile salts and crystal violet will inhibit the growth of most Gram positive bacteria, making MacConkey agar selective. Lactose, a fermentable carbohydrate, and neutral red, a pH indicator, are added to differentiate the lactose positive coliforms from the potentially pathogenic lactose non-fermenters. When lactose is fermented, acid products lower the pH below 6.8, with the resulting colonial growth turning pinkish-red. If an organism is unable to ferment lactose, the colonies will be colorless. Bile salts mixture and crystal violet are the selective agents, inhibiting Gram positive cocci and allowing Gram negative organisms to grow. Sodium Chloride maintains the osmotic environment. Agar and a proprietary polymer are the solidifying agents.

    Eosin Methylene Blue Agar-Levine (EMB) - Differential and slightly selective medium for the isolation, cultivation and differentiation of Gram negative enteric microorganisms (bacilli) isolated from both clinical and nonclinical specimens. Gram (+) bacteria are inhibited. It is widely used for the examination of materials for the presence of coliforms. Eosin Methylene Blue Agar, abbreviated EMB, was developed by Holt-Harris and Teague. This formula contains lactose and sucrose with two indicator dyes, Eosin Y and Methylene Blue. Levine modified the formula by removing sucrose and doubling the concentration of lactose.

    Application

    Enumeration of Gram (-) rods, in particular, coliforms, and specifically, E. coli.

  • BioPaddle® Total Coliform Activity

    Order Code 5846

    Fast and reliable cultivation and enumeration of coliform bacteria. Includes 5553/Nutrient TTC/MacConkey BioPaddles® (10 paddles) described above. Activity also includes a Teacher Resource CD with a complete coliform test activity, Teacher and Student Guides, PowerPoint and iPad/iPod formatted material that provides helpful background information on coliforms, field testing, Fecal Coliform Count, Total Viable Count (TVC) and Total Coliform Count (TCC) enumeration. Can be used as a demonstration or a classroom activity. Additional BioPaddles are available. Also includes a Free LaMotte BioPaddles® Colony ID™ Lite App.

    NOTE: Dated material. Specifiy ship date. Orders may be placed in advance. Orders shipped from Maryland.

  • AP® Environmental Science Water Quality Assessment Curriculum Module

    Order Code 5845

    The AP® Environmental Science WATER QUALITY ASSESSMENT MODULE includes an extensive curriculum that uses the exploration of the Water Quality Index to teach students STEM-based skills. Classroom and field activities satisfy Section VI (Water Pollution) of the AP® Environmental Topics Outline. Includes 10 Nutrient—TTC/MacConkey BioPaddles® (Code 5553) for coliform testing, Salt/TDS/Temp Tracer (Code 1749-01) for temperature and TDS determination and Teacher Resource CD. Also includes a Free LaMotte BioPaddles® Colony ID™ Lite App. Module is designed to be used with the Water Quality Educator (Code 5870-01)

    Teacher Resource CD contains: Teacher/Student Guides, water quality field procedures, STEM-based activities, PowerPoint presentations and Quick Time iPad/iPod videos for Watershed Ecology, WQI, Water Pollution, and Statistical Analysis! Also includes a Free LaMotte BioPaddles® Colony ID™ Lite App.

  • AP® The Water Quality Assessment Package

    Order Code 5845-PKG

    Combines the Water Quality Educator (Code 5870-01) and the AP® Environmental Water Quality Assessment Curriculum Module (Code 5845). Also includes a Free LaMotte BioPaddles® Colony ID™ Lite App which lets users compare colony examples on BioPaddle® media type from 5 microhabitats (air, water, soil, surface and food).

  • BioPaddles® Colony ID™ lite

    Order Code

    The BioPaddles® Colony ID™ lite app allows the user to presumptively identify microbe colonies on LaMotte BioPaddles® by comparing them to a library of 80 standard images covering 5 microhabitats (AIR, WATER, SOIL, SURFACE, and FOOD) and 9 paddle agar types. The image series includes side views, and top views at the colony and cell level.

    Resource materials include instructions for use of the BioPaddles®, detailed fact sheets for each agar type and microbe genera (along with selected species) – including microhabitat preferences, procedures for common microbiological techniques, and examples of microbe growth on BioPaddles® from a variety of microhabitat sources.

  • BioPaddles® Colony ID™

    Order Code

    The BioPaddles® Colony ID™ includes over 250 standard images covering 30+ microbe genera in five microhabitats (AIR, WATER, SOIL, SURFACE, and FOOD) on 9 paddle agar types. The image series includes side views and top views at the colony and cell level.

    New camera and reporting functions allow the user to import digital images and compare their samples to know standards. It also allows the user to create and email reports.

  • BioPaddles Colony ID

    Order Code BioPaddles® Colony ID™

    The BioPaddles® Colony ID™ includes over 250 standard images covering 30+ microbe genera in five microhabitats (AIR, WATER, SOIL, SURFACE, and FOOD) on 9 paddle agar types. The image series includes side views and top views at the colony and cell level.

    New camera and reporting functions allow the user to import digital images and compare their samples to know standards. It also allows the user to create and email reports.